Table of Contents
Inoculating Grain Jars from Mycelium on Agar Step 1: Gather Your Materials. In order to make first generation grain spawn, you first need to get some properly prepared and sterilized grain. Step 2: Set Up in a Clean Environment. Step 3: Grab some mycelium. Step 4: Inoculate. Step 5: Shake the Jar. Step 6: Allow to Colonize.
How do you inoculate your substrate?
Spread the substrate on a large clean surface wiped with alcohol, add spawn, mix well, then place in containers. Or add the straw and spawn in alternating layers. The recommended percentage seems to differ from source to source but most recommend 10 to 20% spawn to substrate.
How many jars will a spore syringe inoculate?
The standard is 1cc per jar, so ten jars. If you go with less, you risk not getting full colonization or having some competing contaminant overpower or contaminate the jar. I will say that if you’re wanting a small amount for personal use, this method works fine.
How long does it take for mycelium to colonize substrate?
Generally speaking the optimal time is 16 to 19 days, but there are certainly also exceptions. If the compost is colonised in the growing room, it is left there so the mycelium stays intact.
How do you transfer mycelium to substrate?
Open the jars or the autoclave bags, spear the mycelium slices with the scalpel and transfer them carefully onto the sterilised substrate. Close the jar/bag tight after this is done. Now distribute the mycelium pieces evenly by shaking the jar or bag to achieve fast and equal colonisation.
Can you make liquid culture from spore syringe?
Turn one spore syringe into 10 or more! Simply inject 3-5 cc’s of your favorite spores in the culture jar and watch it transform into liquid mycelium. In a few days it will be ready to inject into any substrate jar or spawn bag. 10 ounce Jar of liquid culture solution, pressure sterilized and ready to use.
Where do jars go after inoculation?
Put your newly inoculated jars on a shelf at room temperature and away from direct sunlight. There is no need to “incubate” grain at warm temperatures in the dark, since the mycelium will grow happily in normal room temperature conditions.
How can I speed up mycelium growth?
How to Boost Mycelium Growth Ensure that the medium in which the mushroom is being grown is sterile. Use only fresh agar agar to start the mycelium in this type of growing medium. Plan the correct location for the mushrooms. Keep all wildlife and some insects away from the growing area.
How much water do I add to a mushroom substrate?
No more than one drop of water should come out. Once prepared, the straw can be inoculated (planted). This process should happen indoors in a clean area with minimal traffic. Use 5-10% of the wet weight of the straw in grain or sawdust spawn: a 20 lb.
How do you grow mycelium in a jar?
Substrate Preparation for 1 Dozen Jars Prepare the jars. Hammer four holes through each lid, using a 1/8-inch (3 mm) nail. Mix the substrate. Fill the jars. Place the lids. Sterilize the jars. Inoculate the substrate. Incubate. Dunking.
Does mycelium need oxygen?
During their incubation period mycelium can actually tolerate high levels of CO2 but require respiration so as to not promote bacteria who can tolerate no oxygen.
How do you make a fruiting chamber?
STEP 1: Mark Out The Holes. STEP 2: Drill The Holes. STEP 3: Fill The Bottom With Perlite. STEP 4: Soak Your Perlite. STEP 5: Place Your Mushroom Block in the Fruiting Chamber.
Can you use too much spore solution?
Gently squeeze out about 1.0-1.5 cc of spore solution into each jar, splitting up the amount if you inject through more than one hole. Also be careful of using too much spore solution. With spore syringes it can be easy to accidentally push the plunger on the syringe too forcefully and dump out way too much solution.
What happens if you use too much spore solution?
There is also the issue of possibly throwing off the water content in your jar(too wet) by injecting in too much solution. P.F. has commented in the past that his slower colonizing jars(low spore count innoculations) seem to fruit a lot better.
How long will spores last in a syringe?
What’s the Best Way to Store Mushroom Spores? The best place to store a spore syringe is in your refrigerator. We recommend studying our spores as soon as possible after you receive them, but as we discussed above, you can expect refrigerated syringes to last for up to 30 days and often longer.
What temperature does mycelium grow best?
Growing mycelium should be kept in an ideal temperature range. For example, P. cubensis colonizes most rapidly between 75-80°F (24-27°C). Temperatures higher than this range may kill the mycelium and encourage growth of contaminants, and temperatures lower than this range may slow down colonization.
Should you shake grain jars?
Since we use jars, the agar sticks to the side of the jar. So instead of shaking the agar into the jar, we leave it on top. In this image you can see how the mycelium grows through the agar and starts to colonize the grain. When the colonization looks like this, we shake the jar for the first time.
How many spores does a substrate have?
You will only require drops of 3 milliliters of the spore solution to colonize 1.05 quart or 1 liter of the substrate. One syringe has 20 ml of the mushroom spore solution which is enough to inoculate between 6 and 7 liters of substrate. Drop a few milliliters in each corner of the substrate and everything is set.
