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Hold the syringe with the cap pointing up. Unscrew the the cap from the spore syringe by turning it counter clockwise. Remove the needle from it’s sterile package (leave the protection cap on the needle) and attach it to the spore syringe. Before use shake the spore syringe for a few seconds.
How do you inoculate with a spore syringe?
Shake the syringe to dispense the spores equally. Now put the needle into the bag (at the disinfected area, always above substrate level) and push the spore solution of the same species into the bag. We recommend to use about 10 – 20 ml – that is 1 – 2 syringes – for small bags (2,5 L content).
Can you use too much spore solution?
Gently squeeze out about 1.0-1.5 cc of spore solution into each jar, splitting up the amount if you inject through more than one hole. Also be careful of using too much spore solution. With spore syringes it can be easy to accidentally push the plunger on the syringe too forcefully and dump out way too much solution.
How many jars will a spore syringe inoculate?
The standard is 1cc per jar, so ten jars. If you go with less, you risk not getting full colonization or having some competing contaminant overpower or contaminate the jar. I will say that if you’re wanting a small amount for personal use, this method works fine.
Can you reuse a spore syringe?
Can you sterilize a syringe at home? It isn’t recommended that you reuse syringes.
Can you make liquid culture from spore syringe?
Turn one spore syringe into 10 or more! Simply inject 3-5 cc’s of your favorite spores in the culture jar and watch it transform into liquid mycelium. In a few days it will be ready to inject into any substrate jar or spawn bag. 10 ounce Jar of liquid culture solution, pressure sterilized and ready to use.
How many spore syringes do I need?
Typically each jar requires around 0.75-1.5 cc’s of spore solution. Each Spawn Bag requires 3-5 CC’s per bag.Quick reference on how many spore syringes you need: Simple Kit or 6 Jars 1 Spore Syringe (with leftovers) 24 or 30oz 5grain Jars (3) 1 Spore syringe.
Where do jars go after inoculation?
Put your newly inoculated jars on a shelf at room temperature and away from direct sunlight. There is no need to “incubate” grain at warm temperatures in the dark, since the mycelium will grow happily in normal room temperature conditions.
How do you put a needle on a spore syringe?
Take spore syringe with the cap still on it. Hold the syringe with the cap pointing up. Unscrew the the cap from the spore syringe by turning it counter clockwise. Remove the needle from it’s sterile package (leave the protection cap on the needle) and attach it to the spore syringe.
What is the best grain for mushroom spawn?
Most people consider rye to be the best grain for mushroom spawn. It is nutritionally dense, holds plenty of water, and has relatively small grains with a large surface area. A large surface area is advantageous because it means more inoculation points for the mycelium.
Should I shake spore syringe?
The cap of the sealed syringe is known as a Luer lock. Then, unscrew the lock on the syringe by turning it counterclockwise with your hand. Detach the needle from the sterile package (leave the protective cap on the needle) and attach this needle to the mushroom spore syringe. Shake the spore syringe for a few seconds.
How long do spore syringes last?
The best place to store a spore syringe is in your refrigerator. We recommend studying our spores as soon as possible after you receive them, but as we discussed above, you can expect refrigerated syringes to last for up to 30 days and often longer.
How do you make a mushroom spore print?
Put a drop of water on the top of the cap to help release the spores. Cover the cap with a paper cup or glass and leave for 2-24 hours, depending on the humidity and the freshness of the mushroom. The spores will fall on the paper, foil or glass, making a spore print pattern.
What happens if you inject too much spore solution?
There is also the issue of possibly throwing off the water content in your jar(too wet) by injecting in too much solution. P.F. has commented in the past that his slower colonizing jars(low spore count innoculations) seem to fruit a lot better.
How many spores are in a jar?
We recommend using about 100 ml water and one spore print per jar. The lid of the jars needs a hole of about 1 cm in diameter; via this hole you will later fill the syringes. Fill the jars with water (2/3 of the jar) and put on the lid with the hole during sterilisation.
How long does it take mycelium to colonize a jar?
Generally speaking the optimal time is 16 to 19 days, but there are certainly also exceptions. If the compost is colonised in the growing room, it is left there so the mycelium stays intact.
How much is a bag of spore syringe?
This will vary depending on if you are using spores or a live culture, and also how potent your syringes are. But generally 1-3 ccs of solution is sufficient to inoculate each 1 pound bags. Use 2-6 ccs per 2 pound bag.
How long does it take to inoculate grain spawn?
This culture is usually grown on agar in a petri dish or purchased in a liquid culture syringe, alternatively the spawn can be inoculated with a portion of fully colonised spawn from a prior batch. This grain spawn can take anywhere between 10 days to 3 weeks before fully colonised and ready for use.
How do you sterilize spores?
A process called sterilization destroys spores and bacteria. It is done at high temperature and under high pressure. In health care settings, sterilization of instruments is usually done using a device called an autoclave.
